AnaeroPack™, Anaerobic cultivation sets

About this product

AnaeroPack™ is an easy anaerobic cultivation system without using water or a catalyst. It has enabled anaerobic cultivation to be implemented as easily as for aerobic bacteria.

AnaeroPack™ System is an easy anaerobic cultivation method without using neither water nor catalyst. This is a Japanese product Website translated for your information only. We do not guarantee any specifications or prices herein, as these may be different for product sold outside Japan. We also do not guarantee that product handling as described here in will be in compliance all relevant legal codes in your country. Please rely on materials provided specifically for that purpose at the tine of the sale of the product.

Strong point Easy to Handle No water, no catalyst. Just only to put AnaeroPack™ in jar. Produces suitable atmosphere to grow microorganism. Three types of cultivation, i.e. anaerobic, microaerophilic and CO2 cultivation are easy to prepare. Excellent Cultivation The concentration of CO2 can be selected: 16 % or more for anaerobe, 5-8 % for microaerophiles and Ca 5 % for capnophiles. As to anaerobe, high CO2 concentration helps to enlarge the size of colonies. Very Economical The prices of AnaeroPack™ are reasonable. Easy to cultivate with usual jars. No need to prepare any large equipment. Applicable for any types of Jars Anaerobic cultivation by AnaeroPack™ does not cause the excess change of pressure inside the jar. And does not generate high temperature. Those enable to use any types of jar. Method and Product name Example of cultivation Examples of cultivation using rectangular jars with compartment rectangular jars for AnaeroPack™ can be piled, need no unnecessary space. Example of cultivation using pouch

Anaerobic cultivation Pack-Anaero and Pouch-Anaero work as oxygen absorber-CO2 generator. Only to open the aluminium pack and put the white sachet into the container. No need to add water. No need to use catalyst. for Jar / AnaeroPack™- Anaero For anaerobic cultivation of large quantity. Use with anaerobic jars or W-Zip standing Pouch. The large amount of generating CO2 enables to grow anaerobe faster and to obtain large size of colonies. Especially for the cultivation of Prevotella, Porphyromonas sp., AnaeroPack™ system is reported as best of any other anaerobic methods. (From the reports of 21st and 22nd study meetings of anaerobe infection.) Because the pressure inside the jar is equal to outside of that and it generates no high temperature, they enable to use simple jar. To open and close the jar is very easy. The main ingredient of AnaeroPack™ is organic acid. ※Occasionally black spots may appear on the surface. It does not matter for the culti-vation. You can use as it is. Composition change of the gas caused by AnaeroPack™-Anaero ※The sachet absorbs O2 in the jar, generates about 15% of CO2 or more simultaneously, and produces anaerobic atmosphere. (The concentration of O2 will be zero within 2 hours) ※The color change of oxygen indicator (when the O2 concentration becomes less than 0.1%, it turns to pink) takes longer time (about 2 hours) after the O2 concentration changes to 0%. for Pouch / AnaeroPouch™- Anaero To cultivate up to 2 petri dishes. In case of no-strict anaerobes. 3 plates can be cultirated. Use with W-Zipper Pouch-Bag or Pouch-Bag. Best for small amount of petri dishes. Effective for the transportation of samples. Because the pouch is transparent, the growth of bacteria is visible from outside. The pouch, can be used repeatedly unless it has hole. Cultivation Results Prevotella intermedia Finegoldia magna Fusobacterium necrophorum ※CO2 concentration of anaerobic chamber is 10% OFLX 0.05μg/ml (Photos: Clinical Laboratory of Kanto Teishin Hospital, BMI Inc.) How to use AnaeroPack™-Anaero and AnaeroPouch™-Anaero ※AnaeroPack™-Anearo (Middle size) which can be used for 3.5-liter jar is also available.

Microaerophilic cultivation AnaeroPack™- Anaero, AnaeroPouch™- Anaero work as oxygen absorber-CO2 generator for Microaerophilic cultivation. Only to open the aluminum pack and to put the sachet into the container. No need to add water. No need to use catalyst. for Jar / AnaeroPack™- MicroAero Use with anaerobic jars or W-Zip Standing Pouch with Petri Dish Rack. Gas concentration change by AnaeroPack™-MicroAero O2 concentration 6-12%, CO2 concentration 5-8%. Creates microaerophilic atmosphere within 1 hour! O2: Maintains 6-12% which is suitable for cultivation Microaerophiles such as Campylobacter, Helicobacter will easily become extinct at 15% or higher oxygen concentration. And if less than 5%, their colony size will become smaller. Pack-MicroAero absorbs O2 inside the container quickly and its concentration will become 6-12% within 1 hour. So microaerophiles will grow smoothly. CO2 : Less than 10% which may not affect pH Carbon dioxide concentration which needs to cultivate microaerophiles is more than 5%. Higher CO2 does not make much difference for cultivation results. On the contrary, dissolved CO2 might lower pH of the media and have a bad influence on the susceptibility test of some antibiotics. Pack-MicroAero will prevent this by keeping CO2 concentration about 5-8%. for Pouch / AnaeroPouch™- MicroAero Use with Pouch-Bag or W-Zip Pouch. For 2 petri dishes. To cultivate one dish, put one empty dish to adjust the volume of the air. AnaeroPouch™- MicroAero Use with Pouch-Bag AnaeroPouch™-MicroAero for Thin-Type Jar Caution Campylobacter and Helicobacter prefer high humidity. For example, put water into the jar (about 20cc) by wet cotton wool or wet filter paper. 2. Prevent surface of cultures from drying. Pay attention not to touch sachet and the water put in jar. Rectangular jars with compartments are suitable. How to use AnaeroPack™-MicroAero and AnaeroPouch™-MicroAero

Capnophilic cultivation AnaeroPack™- CO2, AnaeroPouch™-CO2 are CO2 generator for Capnophilic cultivation. Only to open the aluminum pack and to put white sachet into the container. No need to add water. No need to use catalyst. for Jar / AnaeroPack™- CO2 Use with anaerobic Jars or W-Zip Standing Pouch with Petri Dish Rack. Use 1 sachet for 2.5-liter jar. Composition change of the gas caused by AnaeroPack™-CO2 This type of AnaeroPack™ produces a CO2 concentration of about 5%. ※The sachet generates about 5 % of CO2 and promotes growth of capnophiles (e.g. Hemophilus sp., Neisseria sp. etc.). ※Usable for searching the responsible bacteria of RTI, such as Streptococcus sp. And Branhamella catarrhalis. It is also reported that S.pneumoniae, S.milleri group grew better than candle Jars. for Pouch / AnaeroPouch™- CO2 For Pouch-Bag, W-Zip Pouch-Bag and Thin-Type Jar. For 2 petri dishes. Suitable for 2 plates. If only 1 plate is needed,place an empty plate in the pouch to maintain constant volume. How to use AnaeroPouch™ : (Common to anaerobic, microaerophilic and capnophilic) 1 After opening aluminum bag, put sachet into the pouch immediately. 2 Seal the pouch along broken line with clip avoiding wrinkle. Do not contain air in the pouch too much nor too less. (In case of W - Zipper, close carefully.)

Preservation of culture media AnaeroPack™- Keep, AnaeroPouch™- Keep are oxygen absorbers for preservation of culture media. The problems of previous method Unused culture media for anaerobes should be anaerobically stored at cool temperature. Under aerobic atmosphere, oxygen will be dissolved in media which may lead worse grow of anaerobes. By refrigerating culture medium using AnaeroKeep helps the growth of anaerobes when the medium is used. And it enables to preserve the medium longer. for Standing-Pouch/AnaeroPack™- Keep Best suited for storing large numbers of anaerobic culture media. Please use it with standing-Pouch. for Pouch / AnaeroPouch™- Keep Suitable for storing small numbers of anaerobic culture media. Use with Pouch-Bags. Absorbs oxygen in half day to create suitable atmosphere for the preservation of anaerobic media. Anaero-Indicator may turn to pink in half a day or so after O2 concentration becomes zero. about preservation When using Standing-Pouch: Close the upper side of the Pouch by Pouch-Clop by eliminating air. If other containers are used, it could lead to difficult opening of the lids caused by negative pressure, Cannot be used with Rectangular Jars. When using pouch: Up to six plates can be stored. Close with Pouch-Clip by eliminating air. Can be repeatedly open for 2 to 3 times.

Containers Rectangular jar Microplate and rectangular culture dish are also usable for anaerobic cultivation by using the rectangular jar. Jars can be piled in the incubator. Special compartment for Standard, Thin-Type and Large. Thin-type is convenient for the transportation of the samples. By standing shorter side, can be cultivated using test tubes. List of Rectangle Jars How to use jars with special compartment "Standard", Thin-type" and "Large" have compartments. Suggested usage is as below: A Place the sachets. B Put 20ml of water humidity for anerobic cultivation. Put anaerobic indicator in case of anaerobic cultivation. ※Put the cover on the jar suitably, then set latches. If you set latch first and then cover forcedly, or set latches to not-suitably covered jar, jar and cover might be damaged. Jar materials and their resistance level ※Heat and cold resistances are those of the materials. Distortion may occur by change of pressure inside of the jars. ※Not autoclavable. ※Cannot be used for the cultivation of thermophiles. (Use Standing-Pouch.) Attention Only AnaeroPack™ can be used for these rectangular jars. Sometimes it requires some power to open the cover because the generated CO2 dissolves into the culture, which produces lower pressure inside the jar. In that case, pull one of the corners of the cover strongly. Do not use AnaeroKeep for rectangular jars because the pressure inside the jar becomes lower and the shape of the jar might be distorted slightly. When rubbish sticks to the washer, airtightness goes down. Wash with detergent, or wipe with alcohol occasionally. Standing-Pouch Use with Pouch-Clip for cultivation of up to 14 plates or media preservation. In case of microbic or CO2 cultivation, use with Petri Dish Rack. Examples of cultivation using Standing-Pouch Attention Though disposable, can be used repeatedly unless it has no hole. When using, 1) Pull the flexible end accompany to the folds, 2) Seal by the clip avoiding wrinkles. (If the pressure inside the jar differs from outside, flexible side will adjust the pressure).

Related Products Anaero-indicator (should be refrigerated) Presence of O2 can be checked by the color change Though the change of the indicator's color is reversible, its sensitivity will go down when rensed. So consider as disposable. There are pinholes on the film of oxygen indicator, The color changes by coming and going of oxygen from the pinhole. Use as it is. (Do not take pill from the film.) Be sure to keep under oxygen-free, cool condition. Remainder should be sealed immediately after removing air in the pouch. Good through 6 months under cool condition. Petri dish rack Petri dish rack for rect. jar (standard) Can be used for microplates, petri dishes and rectangular dishes. Can be placed to the Rectangular Jar(Standard) at a time by holding the plates. Made by stainless steel. Capacity of the petri dish rack for rect. jar 10 units of petri dishes 5 units of rectangular dishes or microplates Petri dish rack for Standing-Pouch 12 petri dishes can be held. Example Test Tube Rack [made by stainless steel] Stain-resistant, washable. High durability. Compact Z-shape.Space-saving. Up to 3 x 4 = 12 units of test tubes can be hold. Usable up to the test tubes of diameter 18mm (19mm of opening). Examples of cultivation using rectangular jars(Standard) Clips for sealing the pouches For normal cultivation For the cultivation of thermophiles